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  • ±âÁ¸ ±¤ÇÐ Çö¹Ì°æÀÇ ¾à 2¹è ÀÌ»óÀÇ ÃÊÇØ»ó·Â

N-SIMÀº ±¸Á¶È­ Á¶¸í Çö¹Ì°æ ¹ý(Structured Illumination Microscopy)À» »ç¿ëÇÑ ÃÊÇØ»ó·Â Çö¹Ì°æÀÔ´Ï´Ù. ȹ±âÀûÀΠ¹æ¹ý°ú CFI apochromat TIRF 100¡¿H ´ë¹° ·»Áî(NA=1.49)µî, ´ÏÄÜÀÇ Ã·´Ü ±¤ÇÐ ±â¼ú°úÀÇ Á¶ÇÕ¿¡ ÀÇÇØ °ø°£ ºÐÇØ´ÉÀ» ±âÁ¸ ±¤ÇÐ Çö¹Ì°æÀÇ ¾à 2¹è ÀÌ»ó(¾à 100nm*)±îÁö Çâ»ó½ÃÄÑ ¶óÀ̺꼿 ³»ÀÇ ¹Ì¼¼ ±¸Á¶¿Í ¹°ÁúÀÇ ¿òÁ÷ÀÓ ÃÊÇØ»óµµ °¡½ÃÈ­¸¦ ½ÇÇöÇß½À´Ï´Ù.

(*TIRF-SIM(488nmÀÇ °æ¿ì)¿¡¼­´Â 85nm±îÁö) 

YFP·Î Ç¥½Ä µÈ B16Á¾¾ç ¼¼Æ÷ÀÇ ¹Ì¼¼ ¼Ò°ü
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Ãëµæ ½Ã°£:¾à 1.8ÃÊ/¸Å(µ¿¿µ»ó)
ÃÔ¿µ Çù·Â:µ¶¸³ ÇàÁ¤ ¹ýÀÎ ÀÌÈ­ÇÐ ¿¬±¸¼Ò »ý¸í ½Ã½ºÅÛ ¿¬±¸ ¼¾ÅÍ ¼¼Æ÷ ±Ø¼º Åë¾î ¿¬±¸ ÆÀ ¿ÀÄ«´Ù ¾ß½º½Ã ¼±»ý´Ô

 

 

                                                                          N-SIMÈ­»ó(Slice 3D-SIM¸ðµå)                ±âÁ¸ ±¤ÇÐ Çö¹Ì°æ »çÁø

 


GFP·Î Ç¥½Ä µÈ HeLa»ý ¼¼Æ÷ÀÇ ¼ÒÆ÷ü
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                                                                          N-SIMÈ­»ó(Slice 3D-SIM¸ðµå)                ±âÁ¸ ±¤ÇÐ Çö¹Ì°æ »çÁø


  • ÃÊÇØ»ó·Â À̹ÌÁö¸¦ 0.6ÃÊ/ÀåÀ¸·Î ÃÔ¿µ

ÃÊÇØ»ó·Â Çö¹Ì°æÀ¸·Î °í¼ÓÀÇ(0.6ÃÊ/Àå) ¿¬¼Ó À̹Ì¡ÀÌ °¡´É.

¶óÀ̺꼿À̳ª Á¶Á÷ ³»ÀÇ ½Ç½Ã°£ º¯È­¸¦ ÃßÀûÇÒ ¼ö ÀÖ½À´Ï´Ù(TIRF-SIM/2D-SIM¸ðµå¿¡¼­ °¡Àå ºü¸¥ °æ¿ì. Slice 3D-SIM¸ðµåÀÇ °æ¿ìµµ ÃÖ°í ¼Óµµ 1ÃÊ·Î À̹Ì¡ °¡´É).

Mito-Tracker red·Î Ç¥½Ä µÈ ¹ÌÅäÄܵ帮¾Æ. ¶óÀ̺꼿 ÃÊÇØ»ó·Â °üÂû·Î, ±âÁ¸º¸´Ùق¹èÀÇ Çػ󵵿¡¼­ ¹ÌÅäÄܵ帮¾ÆÀÇ ¿òÁ÷ÀÓÀÌ ¹àÇôÁö°í ³»ºÎÀÇ Å©¸®½ºÅ¸(cristae)±îÁö ¼±¸íÈ÷ °üÂûÇÒ ¼ö ÀÖ½À´Ï´Ù.

Slice 3D-SIM¸ðµå
´ë¹° ·»Áî:CFI apochromat TIRF 100xH(NA1.49)
Ãëµæ ½Ã°£ °£°Ý:¾à 1ÃÊ(µ¿¿µ»ó)


  • TIRF-SIM ¸ðµå

½Å°³¹ßÀÇ TIRF-SIM´Â, ÀÌÀüÀÇ TIRF Çö¹Ì°æº¸´Ù ³ôÀº ºÐÇصµ·Î Àü¹Ý»ç Á¶¸í Çü±¤ °üÂûÀÌ °¡´ÉÇÏ°í, ¼¼Æ÷¸· ±¸Á¶¸¦ º¸´Ù »ó¼¼ÇÏ°Ô °üÂûÇÒ ¼ö ÀÖ½À´Ï´Ù.

 

±¤ÇÐÇö¹Ì°æÀ¸·Î °üÂû                        TIRF-SIM


  • 3D-SIM ¸ðµå

2°¡Áö ¸ðµå¸¦ Áغñ.

Slice 3D-SIM¸ðµå¿¡¼­´Â ZÃà ¹æÇâÀÇ ÃÊÇØ»ó·Â À̹Ì¡¿¡ ÀÇÇØ ±¤ÇÐ ÀýÆí ÈÄ ¾à 300nmÀÇ ¼½¼Å´× À̹ÌÁö°¡ »ý¼ºµË´Ï´Ù.

Stack 3D-SIM¸ðµå¿¡¼­´Â Slice 3D-SIM¸ðµåº¸´Ù µÎ²¨¿î »ùÇÃÀ» º¸´Ù ³ôÀº ÄÜÆ®¶ó½ºÆ®¿¡¼­ À̹Ì¡ÀÌ °¡´ÉÇÕ´Ï´Ù.

         

N-SIMÈ­»ó(Slice 3D-SIM¸ðµå)                         ±âÁ¸ ±¤ÇÐ Çö¹Ì°æ »çÁø

¸· »ö¼Ò Nile Red(»¡°­)À¸·Î ¿°»öÇØ GFP(³ì»ö)°ú À¶ÇÕÇÑ ¼¼Æ÷ ºÐ¿­ ´Ü¹éÁú DivIVA¸¦ ¹ßÇöÇÑ °íÃÊ±Õ ¹ÚÅ׸®¾Æ
N-SIM¿¡ ÀÇÇØ ¼¼Æ÷ ºÐ¿­ ÁßÀÇ ´Ü¹éÁúÀÇ »óŸ¦ Á¤È®È÷ °¡½ÃÈ­ÇÒ ¼ö ÀÖ½À´Ï´Ù.
ÃÔ¿µ Çù·Â:Drs. Henrik Strahl and Leendert Hamoen, Centre for Bacterial Cell Biology, Newcastle University

          

Stack 3D-SIM¸ðµå(Volume view)                     Stack 3D-SIM¸ðµå(Maximum projection)

Ç× Äɶóƾ Áß°£ Å©±â Çʶó¸àÆ® Ç×ü·Î Ç¥½ÃÇÏ´Â, Alexa 488Ç¥Áö 2Â÷ Ç×ü·Î ¿°»öÇÑ ¸¶¿ì½ºÀÇ Äɶóƾ »ý¼º ¼¼Æ÷
ÃÔ¿µ Çù·Â:Dr. Reinhard Windoffer, RWTH Aachen University


  • 5°³ÀÇ ·¹ÀÌÀú¸¦ ÀÌ¿ëÇÑ ´Ù»ö ÃÊÇØ»ó·Â À̹Ì¡ÀÌ °¡´É

LU5 N-SIM 5·¹ÀÌÀú ¸ðµâ¿¡´Â ÃÖ´ë 5°³ÀÇ ·¹ÀÌÀú°¡ žÀç °¡´ÉÇÏ°í, ´Ù»öÀÇ ÃÊÇØÀ̹Ì¡ÀÌ ½Ç½ÃÇÒ ¼ö ÀÖ½À´Ï´Ù. º¹¼öÀÇ ±¸Á¶Ã¼ÀÇ À§Ä¡ °ü°è, ´Ù¸¥ ¹°ÁúÀÇ »ó°ü, ¿òÁ÷ÀÓ µîÀÌ °üÂû °¡´ÉÇÕ´Ï´Ù.

VEGF½ÅÈ£ÀÇ Ç¥Àû ´Ü¹éÁú(Cy3)°ú ±× Ubiquitin E3¿¬°á È¿¼Ò(FITC)ÀÇ À̹ÌÁö.
nuclear bodyÀÇ ±¸Á¶¸¦ ´õ¿í »ó¼¼È÷ °üÂûÇÒ ¼ö ÀÖ½À´Ï´Ù.
´ë¹° ·»Áî:CFI apochromat TIRF 100xH(NA1.49)
ÃÔ¿µ Çù·Â:¿¡È÷¸Þ ´ëÇÐ ´ëÇпø ÀÇÇÐ°è ¿¬±¸°ú ¿À¿À´©Å° È÷µ¥Å¸Ä« ¼±»ý´Ô, È÷°¡½Ã¾ß¸¶ ½Ã°ÔÅ° ¼±»ý´Ô

         

Slice 3D-SIM¸ðµå                                     3D Volume À̹ÌÁö


  • ±¸Á¶È­ Á¶¸í Çö¹Ì°æ ¹ý(Structured Illumination Microscopy)ÀÇ ¿ø¸®

½ºÆ®¶óÀÌÇÁ Á¶¸íÀ¸·Î ¸¸µé¾îÁø ¸ð¾Æ·¹ ¹«´Ì¸¦ Àаí È­»ó 󸮿¡¼­ ±¸Á¶¸¦ º¹¿ø

¾î´À ƯÁ¤ÀÇ ³ó´ã ÆÐÅÏÀ¸·Î Á¶¸íÇÏ´Â °ÍÀ» "±¸Á¶È­ Á¶¸í"À̶ó°í Çϴµ¥, Ç¥º»ÀÇ ¹Ì¼¼ ±¸Á¶ À§¿¡ ½ºÆ®¶óÀÌÇÁÀÇ Á¶¸í ÆÐÅÏÀ» °ÅµìÇÏ¸é ¸ð¾Æ·¹¶ó°í ºÒ¸®´Â ±½Àº ÁÙ¹«´Ì°¡ ³ªÅ¸³³´Ï´Ù. ¸ð¾Æ·¹¿¡´Â Ç¥º»ÀÇ ¼¼ºÎ±¸Á¶Á¤º¸°¡ Æ÷ÇԵǾî Àֱ⠶§¹®¿¡ ¸ð¾Æ·¹¸¦ ÃÔ¿µÇÏ¿© À̹ÌÁö ¿¬»ê¿¡ ÀÇÇØ ¿ø·¡ÀÇ ±¸Á¶¸¦ º¹¿øÇÏ´Â °ÍÀ¸·Î Çö¹Ì°æÀÇ ºÐÇØ´ÉÀ» ³ÑÀº ¼¼¼¼ÇÑ ±¸Á¶ À̹Ì¡ÀÌ °¡´ÉÇÕ´Ï´Ù.

ÀÌ°ÍÀÌ ±¸Á¶È­ Á¶¸í Çö¹Ì°æ ¹ýÀÔ´Ï´Ù.

°ø°£ ÁÖÆļö ÆÐÅÏ Á¶¸íÀ» Á¶»çÇÔÀ¸·Î½á ¹Ì¼¼ ±¸Á¶ÀÇ ÃÊÇØ»ó·Â Á¤º¸¸¦ "¸ð¾Æ·¹ ¹«´Ì"·Î ¾òÀ» ¼ö ÀÖ½À´Ï´Ù.

 

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±¸Á¶È­ Á¶¸í¿¡¼­ ¾òÀº ¸ð¾Æ·¹ À̹ÌÁö¿¡´Â »ùÇà ³»ÀÇ ¹Ì¼¼±¸Á¶Á¤º¸°¡ Æ÷ÇԵǾî ÀÖ½À´Ï´Ù.

±¸Á¶È­ Á¶¸íµÈ 1ÀåÀÇ Ãëµæ À̹ÌÁö¿¡¼­´Â Á¤º¸°¡ ºÎÁ·Çϱ⠶§¹®¿¡ À§»ó°ú ¹æÇâÀ» ¹Ù²ã ¿©·¯ÀåÀÇ À̹ÌÁö¸¦ ÃÔ¿µÇÑ ÈÄ ¸ð¾Æ·¹ ¹«´Ì¿¡¼­ À̹ÌÁö ¿¬»ê¿¡ ÀÇÇÑ ¹Ì¼¼ ±¸Á¶¸¦ ÃßÃâÇÏ¿© ±âÁ¸ÀÇ ¾à 2¹èÀÇ ÇØ»óµµÀÇ À̹ÌÁö¸¦ ±¸ÃàÇÕ´Ï´Ù.


  • ÃÊÇØ »ó Çö¹Ì°æ¿¡ ÃÖÀûÀÇ ´ë¹° ·»Áî

SR(ÃÊÇØ»ó·Â)´ë¹° ·»Áî´Â ±âÁ¸ÀÇ Çö¹Ì°æÀÇ ÇѰ踦 ³Ñ´Â ¼º´ÉÀ» ½ÇÇöÇϱâ À§ÇØ °³¹ßµÇ¾ú½À´Ï´Ù. ÃÖ½ÅÀÇ ±¤ÇÐ ¼³°è¿Í ¼±º°µÈ ±¤ÇÐ À¯¸®¿¡ ÀÇÇØ ±¸¸é ¼öÂ÷¿Í ½Ç¸°´õÇü ¼öÂ÷¸¦ ±ØÇѱîÁö Àú°¨½ÃŲ ±¤ÇÐ ¼º´ÉÀ» ½ÇÇöÇß½À´Ï´Ù.

  CFI SR Plan Apochromat IR 60x WI         CFI SR Apochromat TIRF 100xH

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